Введение

• Цель: Extraction of stool RNA.
• Совместимость образцов: Suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples.
• Технологии: Utilizes silica gel column purification technology and an original solution system.
• Key Feature: Effectively removes humic acid and other inhibitory factors present in stool samples.
• Приложения: The purified RNA can be directly used in RT-ПЦР, Северная гибридизация, и другие эксперименты.

Технические характеристики

Принцип

• Principle of HiPure Silica Gel Column: Utilizes a high binding ability glass fiber filter membrane as the substrate. Under high concentrations of ionizing agents (например, Guanidinium chloride or гуанидин изотиоцианат), nucleic acids are adsorbed through hydrogen bonding and electrostatic interactions, while proteins and other impurities are not adsorbed and removed. Впоследствии, the filter membrane with adsorbed nucleic acids is washed to remove proteins and salts. Окончательно, low salt buffer solution (например, Буфер ТЕ) or water is used to elute the purified nucleic acids, resulting in high purity suitable for downstream experiments.
• Образец обработки: Stool samples are homogenized in the lysis solution, then further lysed in a high-temperature water bath to release RNA into the solution. Chloroform extraction is employed to remove genomic DNA and impurities. The supernatant is transferred to an alcohol-free binding solution, followed by RNA purification through a column. Окончательно, RNA is eluted using RNase Free Water.
• Приложения: The purified RNA is suitable for various downstream experiments such as PCR, Southern hybridization, and enzyme digestion.

Преимущества

• Высокая чистота: Utilizes a unique adsorbent for more efficient removal of inhibitors, ensuring high purity of extracted RNA.
• High Concentration: Achieves maximum extraction of total RNA from stool samples due to its high concentration, maximizing RNA yield.
• Чувствительный: Capable of purifying RNA at the level of picograms (PG), ensuring sensitivity in downstream applications.

Содержимое комплекта

Хранение и стабильность

• Условия хранения: Kit components can be stored at room temperature (15–25°С) and remain stable for 18 месяцев в таких условиях.
• Буферный уровень звукового давления: May form precipitates at low temperatures. Dissolve precipitates by placing the Buffer SPL in a 55°C water bath.
• Buffer PHC: После получения продукта, store Buffer PHC at 2-8°C.