HiPure Midi Kit C циркулирующей ДНК (Вакуумный протокол)

Корпулирующий гибельный комплект ДНК MIDI, разработан для эффективной очистки коротких, Бесклеточная ДНК (вкДНК) molecules from human biofluids for clinical use:

• Цель:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Совместимость образцов:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000б.п.) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Clinical Applications: The purified cfDNA is suitable for use in in vitro diagnostic (ИВД) tests approved for clinical purposes.

Преимущества:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Широкая совместимость образцов: Works effectively with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows for processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (НИПТ), and liquid biopsy analyses, ultimately aiding in patient diagnosis and management.

Технические характеристики

Принцип

This product offers a fast and reliable method for purifying DNA using silica column chromatography.

1. Лизис и пищеварение: The sample is treated with a solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The lysate is transferred to a column. The silica membrane in the column captures DNA molecules, while impurities pass through.
3. Мойка: Wash buffers to remove unbound proteins and cellular debris.
4. Элюирование: A low-salt buffer elutes the purified DNA from the silica.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Преимущества

• Высокая доходность — самый оптимальный процесс, свободная ДНК (>50б.п.) можно получить в максимальной степени
• Высокая концентрация — низкий объем элюирования, обеспечение высокой концентрации нуклеиновой кислоты
• Высокая чистота — метод связывания с низким содержанием спирта, полное удаление ингибиторов и белковых загрязнений
• Высокое восстановление — DNA can be recovered at the level of PG by silica gel column purification

Содержимое комплекта

Хранение и стабильность

• Keep it cool: Store Proteinase K and Carrier RNA in the fridge (2-8°С) for best results.
• Room temp is okay (for a while): They can last at room temperature (15-25°С) до 12 недели без проблем.
• Everything else: Store other kit components dry at room temperature (15-25°С), good for at least 18 месяцы.
• Важный: If storing everything cold, не забудьте прогреть буферы перед использованием.

Данные эксперимента