Introduction
- The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
- Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 to 11.0 during nucleic acid extraction.
- Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 to 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
- Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. Typically, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
- This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.
Details
Specifications
Features | Specification | |
Basic content | Recommended application | Nucleic acid extraction |
CAS Numbers | 1185-53-1 | |
Formula | C4H11NO3.HCL | |
Molecular Weight | 157.60 | |
Stability | Unlimited in dry conditions | |
Level | Molecular Biology | |
Appearance | White crystal | |
Purity | ≥99% | |
Transportation conditions | Room temperature | |
Preservation conditions | Room temperature | |
Impurity parameters | Smell | Nothing |
Melting point ℃ | 145-155 | |
Heavymetal | <5 PPM | |
Losson drying | ≤0.09% | |
UV absorption value | Absorbance value @ 230 (5%) | ≤0.01 |
Absorbance value @ 260 (5%) | ≤0.01 | |
Absorbance value @ 280 (5%) | ≤0.01 | |
Absorbance value @ 320 (5%) | ≤0.01 | |
Nucleic acid extraction related | DNase test (0.5M) | Not detected |
RNase test (0.5M) | Not detected | |
PH value (2M) | 4.0-5.0 |
Ordering information
CAT.No. | Product Name | Package |
C11224 | Tris-HCl, TRIS hydrochloride (Molecular Biology) | 1KG |
C11225 | 5KG |
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