Guanidinium thiocyanate

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Molecular Biology Grade, CAS: 593-84-0

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Description

Introduction

The product is the core raw material for nucleic acid extraction after strict screening and quality control. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.

Details

Specifications

FeaturesSpecification
Basic contentRecommended applicationAgarose Gel DNA Purification, DNA/RNA Isolation (Lysis Buffer)
CAS Numbers593-84-0
FormulaCH5N3•HSCN
Molecular Weight118.6
Content99.5%
LevelMolecular Biology
AppearanceWhite powder to crystal
Transportation conditionsRoom temperature, dry
Preservation conditionsAvoid light
Impurity parametersMoisture≤0.3%
Ash≤0.02%
Water insoluble matter≤0.02%
Ammonium≤0.05%
Ammonium dicyanide≤0.1%
Ammonium cyanuric chloride≤0.01%
Iron≤0.1PPM
UV absorption valueAbsorbance value @ 230 (6M)≤20
Absorbance value @ 260 (6M)≤25
Absorbance value @ 280 (6M)≤0.3
Absorbance value @ 320 (6M)≤0.01
Nucleic acid extraction relatedGel DNA PurificationAdopt
RNA IsolationAdopt
DNase test (1M)Not detected
RNase test (1M)Not detected
Purity of saturated solution (6M)Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration)
PH value (1M)5.0-7.0

Ordering information

CAT.No.Product NamePackage
C11103Guanidine Thiocyanate

(Molecular Biology)

5KG
C1110425KG (5KG/Bag, 5Bags/Bucket)

Additional information

Weight0.7 kg
size

Guanidinium thiocyanate 5 KG, Guanidinium thiocyanate 25 KG

brand name

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