Product Overview
- Nile Red is a lipophilic dye commonly used to detect lipid droplets within cells.
- It belongs to the family of lipophilic phenoxazine fluorescent dyes.
- When combined with esters, triglycerides, various fatty acids, and other lipid substances, Nile Red emits fluorescence.
- Under excitation at a wavelength of 543nm, it exhibits intense orange fluorescence (with an emission wavelength of 598nm).
- Additionally, it shows red fluorescence under ultraviolet light.
- Furthermore, it can also be used for protein staining.
Specification
| Property | Value |
|---|---|
| CAS | 7385-67-3 |
| English Name | Nile Red |
| Synonym | Nile Blue A oxazone; Phenoxazone 9 |
| Unit | Bottle |
| Purity | ≥98% |
| Molecular Formula | C20H18N2O2 |
| Molecular Weight | 318.37 |
| Appearance | Green to black solid |
| Storage | Powder: 2-8℃, 2 years; In solvent (mother liquid): -20℃, 1 month; -80℃, 6 months |
| Solubility | Soluble in DMSO |
| EC | EINECS 230-966-0 |
| MDL | MFCD00011639 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| PubChem CID | 65182 |
| SMILES | O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1 |
| Description | Nile Red is a dye commonly used to detect intracellular lipid droplets. |
Preparation of Stock Solution
Prepare a 10 mM stock solution using DMSO. For example: Dissolve 1 mg of Nile Red powder in 314.1 μL of DMSO.
Note:
- Unused stock solution is recommended to be aliquoted and stored at -20°C to avoid repeated freeze-thaw cycles.
- Moisture-absorbed DMSO significantly affects the solubility of the product. Please use freshly opened DMSO.
Preparation of Working Solution
Dilute the stock solution with an appropriate buffer (e.g., serum-free culture medium or PBS) to prepare a working solution of 1-100 μM. Note:
- The final concentration of the working solution is recommended to be optimized according to different cell lines and experimental systems.
- If difficult to dissolve, ultrasound treatment can be used to facilitate dissolution.
Precautions
- Avoid using lipid substances in cell culture media. During staining incubation and observation, avoid strong light exposure to prevent fluorescence quenching.
- For your safety and health, wear lab coat and disposable gloves during operation.
- This product is for research use only. Do not use for pharmaceuticals, clinical diagnosis or treatment, food and cosmetics, etc. Do not store in residential areas.
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