MagPure Universal DNA Kit

$448.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from blood, buffy coat, tissue and other samples
ApplicationsSecond generation sequencing, PCR, real time PCR, etc.
Purification methodPolydisperse magnetic beads
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeAnticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amountWhole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg
Yield0.1 – 50μg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High purity – OD 260 / 280 : 1.7- 1.9, OD 260 / 230 : 1.5 – 2.0
  • Economy – less than 50% of the price of Qiagen and other imported products
  • High yield – most optimal process, ensuring the recovery up to 90%
  • Strong processing ability – samples including animal blood, cultured cells, animal tissues, etc.

Kit Contents

ContentsIVD3102
Purification Times200
MagPure Particles5 ml
Proteinase K100 mg
Protease Dissolve Buffer10 ml
Rnase A40 mg
Buffer ATL60 ml
Buffer AL60 ml
Buffer BD*20 ml
Buffer BW1*110 ml
Elution Buffer30 ml

Storage and Stability

Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.75 kg
brand name

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