MagPure Soil DNA Kit

$239.00$2,138.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 250-500mg soil sample
ApplicationsPCR, southern blot and enzyme digestion, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeSoil
Sample amount250-500mg
Elution volume≥50μl
Time per run≤70 minutes

Principle

This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High concentration – maximum extraction of total DNA from soil samples
  • High purity – purified DNA can be directly used in various downstream applications

Kit Contents

ContentsD635601D635602D635603
Purification Times48 Preps96 Preps5 x 96 Preps
MagPure Particles2.5 ml5 ml22 ml
2ml Bead Tubes4896400
Buffer SOL60 ml100 ml500 ml
Buffer SDS6 ml10 ml50 ml
Reagent DX1 ml1 ml5 ml
Buffer PS10 ml20 ml90 ml
Absorber Solution10 ml20 ml90 ml
Buffer GDP70 ml150 ml2 x 350 ml
Buffer GW1*22 ml44 ml220 ml
Elution Buffer20 ml20 ml60 ml

Storage and Stability

MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.65 kg
size

MagPure Soil DNA Kit 48 preps, MagPure Soil DNA Kit 96 preps, MagPure Soil DNA Kit 480 preps

brand name

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