MagPure Seed DNA Kit

$119.00$995.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed
ApplicationsPCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeConventional economic plant samples
Sample amount50-100mg
Elution volume≥50μl
Time per run≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Safe – require no phenol or chloroform extraction
  • Fast – several samples can be extracted in 60 minutes
  • High purity – high quality DNA, completely remove inhibitors

Kit Contents

ContentsD635201D635202D635203
Purification Times48 Preps96 Preps5 x 96 Preps
MagPure Particles1.7 ml4 ml18 ml
Buffer SOL40 ml90 ml500 ml
Buffer SDS4 ml9 ml50 ml
Buffer MPB40 ml70 ml350 ml
Buffer GW126 ml53 ml220 ml
Elution Buffer10 ml30 ml120 ml

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.75 kg
size

MagPure Seed DNA Kit 48 preps, MagPure Seed DNA Kit 96 preps, MagPure Seed DNA Kit 5 x 96 preps

brand name

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