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MagPure Plasmid EF Mini Kit

$109.00$3,800.00

Shipping USD 45 - Free over USD 300

Isolation of up to 15μg endotoxin-free plasmid DNA from 1-5ml bacterial culture

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
ApplicationsEnzyme digestion, sequencing, PCR and labeling,  etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeConventional plasmid, plasmid≤30KB
Sample amount1-5ml
Elution volume≥50μl
Time per run≤80 minutes

Principle

This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted with an Elution Buffer.

Advantages

  • High purity – purified plasmid can be directly used in sequencing, enzyme digestion PCR, etc.
  • Fast – it takes only 80 minutes to complete the isolation
  • High yield – up to 15μg plasmid can be bound in one column
  • Economic – excellent cost-effectiveness performance

Kit Contents

ContentsP181402P181403P181404
Purification Times100 Preps500 Preps5000 Preps
RNase A10 mg30 mg2 x 160 mg
Buffer P130 ml150 ml2 x 800 ml
Buffer P230 ml150 ml2 x 800 ml
Buffer LEN320 ml80 ml800 ml
Buffer LN490 ml400 ml4 x 980 ml
MagPure Particles3.5 ml17 ml3 x 60 ml

Storage and Stability

RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case, buffers should be redissolved before use. Make sure that all buffers are at room temperature when used. If any precipitates form in the buffers, warm at 37℃ to dissolve. After the addition of RNase A, Buffer P1 is stable for 6 months when stored at

Additional information

Weight0.7 kg
size

MagPure Plasmid EF Mini Kit 100 preps, MagPure Plasmid EF Mini Kit 500 preps, MagPure Plasmid EF Mini Kit 5000 preps

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