HiPure Fastfilter Plasmid Midi Kit

$249.00$895.00

Shipping USD 45 - Free over USD 300

Isolation up to 250μg plasmid DNA from 30-50ml bacterial culture

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  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The HiPure Fastfilter Plasmid DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation up to 250μg plasmid DNA from 30-50ml bacterial culture
ApplicationsEnzyme digestion, sequencing, PCR and labeling,  etc.
Purification methodMidi spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeConventional plasmid, plasmid less than 30KB
Sample amount30-50ml
Yield50-250µg
Elution volume≥300μl
Time per run≤60 minutes
Liquid carrying volume per column4ml
Binding yield of column250µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparationand clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
  • Fast – it takes only 15-60 minutes to complete the isolation
  • High yield – up to 70µg plasmid can be binded in one column

Kit Contents

ContentsP101302P101303
Purification Times25 Preps100 Preps
RNase A10 mg40 mg
Buffer P180 ml300 ml
Buffer P280 ml300 ml
Buffer LEN340 ml150 ml
Buffer GBT60 ml250 ml
Buffer PW160 ml250 ml
Buffer PW220 ml100 ml
Elution Buffer30 ml120 ml
HiPure DNA Midi Columns III25100
Lysate Clear Midi Syringe25100
15 ml Collection Tubes50200

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

NameCAT NOSample amountColumn typeElutio volumeYieldTime per runCentrifuge requirements
HiPure Plasmid Mini KitP10011-5ml1.5ml column30-50μl5–35µg/5ml≤30 minutessmall centrifuge
HiPure Plasmid Mini Kit IIP10025-15ml1.5ml column75-100μl20-80µg/15ml≤30 minutessmall centrifuge
HiPure Plasmid Plus 96 KitP10061-1.5ml96 well plate70-150μl1-15μg/1ml≤60 minutes96 well plate centrifuge
HiPure Fastfilter Plasmid Midi KitP101330-50ml15ml column0.3-0.8ml50–250µg/50ml≤60 minutes15ml centrifuge
HiPure Fastfilter Plasmid Maxi KitP1014100-200ml50ml column0.5-1.5ml0.4–1mg/200ml≤60 minutes50ml centrifuge

Additional information

Weight0.65 kg
size

HiPure Fastfilter Plasmid Midi Kit 25 preps, HiPure Fastfilter Plasmid Midi Kit 100 preps

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