Product Overview
- Suitable for rapid DNA extraction from FFPE samples.
- Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
- High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
- Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation of high pure total DNA from FFPE using high bind beads |
| Applications | PCR and viral DNA detection, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Paraffin-embedded tissue samples |
| Sample amount | 1-6 slices of 10-20μm |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
Principle
Advantages
- High yield – most optimal process, recovery up to 90%
- Economy – less than 50% of the price of Qiagen and other imported products
- High purity – OD 260/280: 1.7-1.9, OD 260/230: 1.5-2.0
Kit Contents
| Contents | D632301D | D632302D |
| Purification Times | 48 Preps | 96 Preps |
| MagPure Particles N | 1.1 ml | 2.5 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer BST1 | 30 ml | 60 ml |
| Buffer BW1 | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
- Proteinase K, RNase A, and MagPure Particles N should be stored at 2-8°C initially.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks doesn’t affect their performance.
- Remaining kit components can be stored at room temperature and remain stable for at least 18 months under these conditions.
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