Introduction
This product offers a convenient and rapid method to purify total DNA from various samples, making it ideal for PCR and Southern blotting applications.
- Fast and Easy Purification: The protocol is streamlined for efficient DNA extraction with minimal hands-on time.
- High-Quality DNA: The system isolates total DNA (genomic, viral, mitochondrial) with high purity, suitable for reliable PCR and Southern blotting.
- Broad Sample Compatibility: The method effectively purifies DNA from a wide range of sources, including:
- Whole blood
- Plasma
- Serum
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Cultured cells
- Pre-packed Reagents: For user convenience, all necessary reagents are pre-plated in a 96-well plate, minimizing preparation time and ensuring consistency across samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 200μl whole blood |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | 200μl |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
Principle
- Sample Lysis and Digestion: The sample undergoes lysis and digestion facilitated by lysate and Protease, releasing DNA into the lysate.
- DNA Adsorption: Magnetic particles and binding solution are added, facilitating the adsorption of DNA onto the surface of magnetic particles. Impurities such as proteins are not adsorbed.
- Washing to Remove Impurities: Adsorbed particles are washed with a washing solution to remove proteins and impurities, ensuring purity.
- Ethanol Wash: Further washing with ethanol removes salts from the particles.
- DNA Elution: DNA is eluted from the particles using an Elution Buffer, providing purified DNA for downstream applications.
Advantages
- High binding force – suitable for handling DNA-rich samples, such as whole blood, buffy coat, concentrated blood, etc.
- Fast – polydisperse magnetic beads, fast magnetic response, and short extraction time
- High purity – the obtained DNA can be directly used for second-generation sequencing, PCR-based detection, gene bank, etc.
- Automatic – saving time and labor and safer
Kit Contents
| Contents | D631101 | D631102 | D631103 |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
| Buffer AL | 15 ml | 30 ml | 120 ml |
| Buffer BD | 5 ml | 10 ml | 50 ml |
| Buffer GW1 | 22 ml | 53 ml | 220 ml |
| Elution Buffer | 15 ml | 30 ml | 100 ml |
Storage and Stability
For optimal performance:
- Store Proteinase K and MagPure Particles at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K and MagPure Particles can be stored for up to 24 weeks at room temperature (15-25°C) without affecting their performance.
Remaining kit components:
- Store all other components dry at room temperature (15-25°C).
- They are stable for at least 18 months under these conditions.
Storing the entire kit:
- The entire kit can be stored at 2-8°C for long-term storage.
- If storing the entire kit cold, ensure buffers are redissolved before use.
- Always warm all buffers to room temperature before using them.
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