Introducción
This product offers a convenient and rapid method to purify total DNA from various samples, making it ideal for PCR and Southern blotting applications.
- Fast and Easy Purification: The protocol is streamlined for efficient DNA extraction with minimal hands-on time.
- ADN de alta calidad: The system isolates total DNA (genómico, viral, mitocondrial) with high purity, suitable for reliable PCR and Southern blotting.
- Compatibilidad de muestra amplia: The method effectively purifies DNA from a wide range of sources, incluido:
- Sangre pura
- Plasma
- Suero
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Células cultivadas
- Pre-packed Reagents: For user convenience, all necessary reagents are pre-plated in a 96-well plate, minimizing preparation time and ensuring consistency across samples.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation total DNA from 200μl whole blood |
| Aplicaciones | PCR, Detección de virus y pernos del sur., etc. |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | Sangre anticoagulante, sangre concentrada, capa leucocitaria, lymphocytes and cultured cells |
| Cantidad de muestra | 200µl |
| Volumen de elución | ≥50 μl |
| Tiempo por carrera | ≤60 minutos |
Principio
- Sample Lysis and Digestion: The sample undergoes lysis and digestion facilitated by lysate and Protease, Liberar el ADN al lisado.
- DNA Adsorption: Magnetic particles and binding solution are added, facilitating the adsorption of DNA onto the surface of magnetic particles. Impurities such as proteins are not adsorbed.
- Washing to Remove Impurities: Adsorbed particles are washed with a washing solution to remove proteins and impurities, Garantizar la pureza.
- Ethanol Wash: Further washing with ethanol removes salts from the particles.
- DNA Elution: DNA is eluted from the particles using an Elution Buffer, providing purified DNA for downstream applications.
Ventajas
- Fuerza de unión alta – Adecuado para manejar muestras ricas en ADN, como la sangre entera, capa leucocitaria, sangre concentrada, etc..
- Rápido – cuentas magnéticas polidispersas, respuesta magnética rápida, y tiempo de extracción corto
- Alta pureza – El ADN obtenido se puede usar directamente para la secuenciación de segunda generación, Detección basada en PCR, banco de genes, etc..
- Automático – saving time and labor and safer
Contenido del kit
| Contenido | D631101 | D631102 | D631103 |
| Tiempos de purificación | 48 | 96 | 480 |
| Partículas MagPure | 1.2 ml | 2.5 ml | 11 ml |
| Proteinasa K | 24 mg | 50 mg | 220 mg |
| Tampón de disolución de proteasa | 1.8 ml | 5 ml | 15 ml |
| Amortiguador AL | 15 ml | 30 ml | 120 ml |
| Buffer BD | 5 ml | 10 ml | 50 ml |
| Memoria intermedia GW1 | 22 ml | 53 ml | 220 ml |
| Tampón de elución | 15 ml | 30 ml | 100 ml |
Almacenamiento y estabilidad
Para un rendimiento óptimo:
- Store Proteinase K and MagPure Particles at 2-8°C (refrigerador) upon arrival.
Acceptable short-term storage:
- Proteinase K and MagPure Particles can be stored for up to 24 weeks at room temperature (15-25°C) without affecting their performance.
Remaining kit components:
- Store all other components dry at room temperature (15-25°C).
- Son estables por al menos 18 meses en estas condiciones.
Storing the entire kit:
- The entire kit can be stored at 2-8°C for long-term storage.
- Si almacena todo el kit frío, ensure buffers are redissolved before use.
- Always warm all buffers to room temperature before using them.
Reseñas
Aún no hay reseñas.