HiPure Viral RNA Kit R4171

$99.00$448.00

Shipping USD 45 - Free over USD 300

Extract viral RNA from 140μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc.

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  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
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Description

Introduction

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

Details

Specifications

FeaturesSpecifications
Main FunctionsExtract viral RNA from 140μl cell-free samples
ApplicationsRT-PCR, Northern hybridization, and various virus detection
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeCell-free body fluid or culture medium
Sample amount140μl
Elution volume≥15μ
Time per run≤25 minutes
Liquid carrying volume per column800μl
Binding yield of column100μg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.

Advantages

  • Fast – several samples can be extracted in 20 minutes by column method
  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Safe – no phenol chloroform extraction required
  • Sensitive – RNA can be recovered at the level of PG
  • High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

ContentsR417102R417103
Purification Times50 Preps250 Preps
HiPure Viral Micro Columns50250
2ml Collection Tubes100500
Buffer VRL50 ml200 ml
Carrier RNA310 µg3 x 310 µg
Buffer VHB*13 ml110 ml
Buffer RW2*20 ml2 x 50 ml
Nuclease Free Water10 ml30 ml

Storage and Stability

Carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions

Additional information

Weight0.85 kg
size

HiPure Viral RNA Kit 50 preps, HiPure Viral RNA Kit 250 preps

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