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HiPure Universal RNA Kit 50 Preps

Original price was: $124.00.Current price is: $109.00.

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Isolation total RNA (include miRNA) from 100mg lipid tissue, tissue, cell, plant, body fluids using columns and MagZol reagent

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The Kit integrates phenol/guanidine-based lysis and silica-membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, plant, and fungal samples, bacteria, cells, and other samples.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation of total RNA (including miRNA) from 100mg lipid tissue, tissue, cell, plant, and body fluids using columns and MagZol reagent
ApplicationsRT-PCR, qRT-PCR, Northern hybridization, second-generation sequencing
Purification methodMini spin column
Purification technologySilica technology, acid phenol/guanidine extraction technology (MagZol pretreatment technology)
Process methodManual (centrifugation or vacuum)
Sample typeAnimal tissue, muscle fiber-containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants, and other biological samples
Sample amountAnimal tissue sample: 1-60mg (spleen ≤ 10mg)

Cultured cells: 5 x 106

Plant leaves: 50-100mg

Yield2-200μg
Elution volume≥50μl
Time per run≤30 minutes(1-24 samples)
Liquid carrying volume per column800µl
Binding yield of column100µg

Principle

Tissue samples (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.

Advantages

  • Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
  • High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast – several samples can be extracted in 30 minutes
  • High applicability – samples including animals, plants, bacteria, cells, etc.
  • High output – enables to processing of large samples and the yield can be up to 200μg

Kit Contents

ContentsR413002D413003
Purification Times50 Preps250 Preps
HiPure RNA Mini Columns50250
2ml Collection Tubes50250
MagZol Reagent60 ml270 ml
Buffer RW150 ml200 ml
Buffer RW2*20 ml50 ml
RNase Free Water10 ml30 ml

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.7 kg

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