HiPure Viral DNA/RNA Kit IVD4173

$249.00

Shipping USD 45 - Free over USD 300

Extract viral RNA/DNA from 200μl plasma/serum samples

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

FeaturesSpecifications
Main FunctionsExtract viral RNA/DNA from non-cell/low cell content biological samples
ApplicationsRT-PCR,PCR,NGS
ProductsViral total nucleic acid
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeCell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount200μl
Yield2-10μg
Elution volume≥30μl
Time per run≤30 minutes
Liquid carrying volume per column800μl
Binding yield of column100μg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages

  • Fast – several samples can be extracted in 20 minutes by column method
  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Safe – no phenol chloroform extraction required
  • Sensitive – DNA/RNA can be recovered at the level of PG
  • High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

ContentsIVD4173
Purification Times100 Preps
HiPure Viral Mini Column100
2ml Collection Tubes200
PK/Carrier RNA50 mg
Protease Dissolve Buffer5 ml
Buffer AL30 ml
Buffer MW1*44 ml
Buffer MW2*50 ml
RNase Free Water15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

Experiment Data

Additional information

Weight0.85 kg

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