HiPure Blood RNA Mini Kit

$149.00$646.00

Shipping USD 45 - Free over USD 300

Isolation of total RNA from 1-1.5ml whole blood

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  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

The Kit provides fast purification of high-quality RNA from whole blood, cells, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation of total RNA from 1-1.5ml whole blood
ApplicationsqPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection
Purification methodMini spin column
Purification technologySilica technology, acid phenol/guanidine extraction technology (MagZol pretreatment technology)
Process methodManual (centrifugation or vacuum)
Sample typeFresh or frozen blood, mammalian blood
Sample amount≤1.5 ml whole blood
Yield2-100μg
Elution volume≥20μl
Liquid carrying volume per column800µl
Binding yield of column100μg

Principle

The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.

Advantages

  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – isolation of several samples can be completed in 20 minutes by using the column purification method
  • Sensitive – RNA can be purified at the level of PG

Kit Contents

ContentsR416102R416103
Purification Times50 Preps250 Preps
HiPure DNA Mini Columns50250
HiPure RNA Mini Columns I50250
2ml Collection Tubes100500
10 x Buffer RBC50 ml3 x 100 ml
RTL Lysis Buffer50 ml250 ml
Buffer RW150 ml250 ml
Buffer RW2*20 ml2 x 50 ml
RNase Free Water10 ml30 ml

Storage and Stability

HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.

Additional information

Weight0.7 kg
size

HiPure Blood RNA Mini Kit 50 preps, HiPure Blood RNA Mini Kit 250 preps

brand name

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