HiPure Universal miRNA Kit

$174.00$746.00

Shipping USD 45 - Free over USD 300

Isolation miRNA and other small RNA molecules(18nt), from cultured cells and tissues. Enrich miRNA, miRNA, and RNA(>200nt) can be purified separately.

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidine-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation miRNA and other small RNA molecules(18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column
ApplicationsRT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeAnimal tissues, adherent cells, suspension cells, bacteria, etc
Sample amountEukaryotic culture cells: ≤ 10^7, Animal tissue:<100mg, Yeast culture cells:<5 x10^7, Bacteria:<10^9
Elution volume≥15μl
Time per run≤40 minutes
Liquid carrying volume per column800µl
Binding yield of column100µg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such asguanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein andother impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

This kit combines acid/guanidine (MagZol) extraction technology with glass fiber filter membrane purification, which can improve the extraction effect of complex samples and samples with low RNA content. After the sample is treated with MagZol reagent and chloroform, the supernatant is added with ethanol to provide appropriate binding conditions, then transferred to the purification column and centrifuged. Macromolecular RNA can be efficiently bound to the membrane. Collect the filtrate containing small RNA, add more ethanol to adjust the binding capacity of small RNA, the pollutants can be efficiently washed away by second cleaning. Finally, the purified RNA was eluted by RNase free water.

Advantages

  • High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast – several samples can be extracted in 40 minutes
  • High applicability – samples including animals, plants, bacteria, cells, etc.
  • High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity

Kit Contents

ContentsR431002R431003
Purification Times50 Preps250 Preps
HiPure RNA Mini Columns1002 x 250
2ml Collection Tubes1002 x 250
MagZol Reagent60 ml270 ml
Buffer RWC20 ml80 ml
Buffer RW2*20 ml2 x 50 ml
RNase Free Water10 ml30 ml

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Additional information

Weight0.85 kg
size

HiPure Universal miRNA Kit 50 preps, HiPure Universal miRNA Kit 250 preps

Reviews (0)

Reviews

There are no reviews yet.

Be the first to review “HiPure Universal miRNA Kit”

Your email address will not be published. Required fields are marked *

Attachment

Shopping Cart
Scroll to Top