- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 minutes.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
Specifications
| Features | Specifications |
| Main Functions | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Soil, stool, other environmental samples |
| Sample amount | Soil: 200-500mg
Stool: 50-100mg Other environmental samples: 100-500mg |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Principle
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, yeast, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
Advantages
- Fast – several samples can be extracted in 40 minutes (after digestion)
- High purity – the purified DNA can be directly used in various downstream applications
- Good repeatability – silica technology can obtain ideal results every time
- High recovery – DNA can be recovered at the level of PG
Kit Contents
| Contents | D314401 | D314402 | D314403 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| HiPure DNA Plate | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ml Bead Tubes | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 x 900 ml |
| Buffer SDS | 10 ml | 36 ml | 180 ml |
| Reagent DX | 1 ml | 1.8 ml | 9 ml |
| Buffer PS | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| Buffer GDP | 150 ml | 500 ml | 3 x 900 ml |
| Buffer GW2* | 50 ml | 100 ml | 4 x 200 ml |
| Buffer AE | 30 ml | 120 ml | 500 ml |
Storage and Stability
- Absorber Solution Storage: Upon arrival, the Absorber Solution should be stored at 2-8°C. However, it can also be stored at room temperature (15-25°C) for short-term periods (up to 24 weeks) without affecting its performance.
- Remaining Kit Components: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 months under these conditions, ensuring their efficacy and performance over an extended period.
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