HiPure HP Plant DNA Kit

$99.00$448.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 150 mg plant and fungal tissue
ApplicationsPCR, SSR, AFLP, RAPD and southern blot, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeFresh / frozen plant samples, dried plant / seed samples
Sample amountFresh / frozen plant samples: 50-150 mg

Dried plant / seed samples: 15-40 mg

Elution volume≥40μl
Time per run30-50 minutes
Liquid carrying volume per column800μl
Binding yield of column100μg

Principle

This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • Broad spectrum – suitable for extracting DNA from various plant samples
  • Fast – several samples can be extracted in 30 minutes by silica technology
  • Good repeatability – silica gel column purification technology can obtain ideal results every time

Kit Contents

ContentsD318702D318703
Purification Times50 Preps250 Preps
Buffer PAL60 ml200 ml
Buffer GWP60 ml200 ml
Buffer DW130 ml150 ml
Buffer GW2*20 ml50 ml
Buffer AE20 ml60 ml
HiPure DNA Mini Columns II50250
2 ml Collection Tubes50250

Storage and Stability

This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

NameCAT NOFresh / frozen tissue amountDried tissue amountColumn typeElution volumeYield (muscle)
HiPure HP Plant DNA KitD3187150mg40mg1.5ml column50 – 100μl3-70μg
HiPure HP Plant DNA Maxi KitD31635g1g50ml column0.7 – 3ml75-1250μg
HiPure SF Plant DNA KitD3164100mg20mg1.5ml column50 – 100μl3-50μg
HiPure SF Plant DNA 96 KitD316750mg15mg96 well plate75 – 150μl3-30μg

Additional information

Weight0.75 kg
size

HiPure HP Plant DNA Kit 50 preps, HiPure HP Plant DNA Kit 250 preps

brand name

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