HiPure Gel DNA Mini Kit

$60.00$150.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
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Description

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

FeaturesSpecifications
Main FunctionsRecover DNA fragments between 60bp-10kbp from agarose gel
ApplicationsPCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeAgarose gel, PCR products, enzyme products
Sample amountAgarose gel: ≤500mg
Recovery≥80%
Elution volume≥15μl
≤20 minutes(1-24 samples)
Liquid carrying volume per column800µl
Binding yield of column35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency – ≥80% DNA recovery
  • General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
  • Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

ContentsD211102D211103
Purification Times100 Preps250 Preps
Buffer GDP120 ml250 ml
Buffer DW250 ml2 x 50 ml
Elution Buffer20 ml30 ml
HiPure DNA Mini Columns II100250
2 ml Collection Tubes100250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

Additional information

Weight0.65 kg
size

HiPure Gel DNA Mini Kit 100 preps, HiPure Gel DNA Mini Kit 250 preps

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