HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
| Features | Specifications |
| Main Functions | DNA Clean Up from agarose Gel and Reactions |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Micro spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>Agarose gel: ≤300mg DNA products: <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤300µl (<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤5µg) |
| Recovery | 100-5000bp：85%, 5-10kb：60-70% |
| Elution volume | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥10μl |
| Time per run | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤30 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
- High recovery efficiency – up to 80% DNA recovery
- Low elution volume – elution volume can be as low as 10μl
- General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
- Fast – isolation can be completed in 10-15 minutes by column gel method
Kit Contents
| Contents | D211002 | D211003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GDP | 60 ml | 125 ml |
| Buffer DW1 | 40 ml | 90 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 20 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
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