MagZol 시약

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and 구아니딘 이소티오시아네이트, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100mg) 그리고 세포 (<5 X10⁶), and large quantities of tissue (up to 1g) 그리고 세포 (<10⁸), of human, 동물, 식물, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, 폴리(ㅏ) + 선택, 시험 관내 번역, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

세부

명세서

장점

• Extensive – suitable for various kinds of biological samples, including animals, 식물, 배양된 세포, 박테리아, 등.
• 높은 수율 – efficient cleavage releases more RNA
• 고순도 – purified RNA is suitable for various downstream applications
• Flexible – sample amount can be adjusted according to the demand
• High cost performance compared with similar products

보관 및 안정성

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. 하지만, 단기 보관 (까지 12 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다.

실험 데이터