MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidin isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) dan sel (<5 X106), and large quantities of tissue (up to 1g) dan sel (<108), of human, hewan, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + pilihan, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | RNA isolation solvent (substitution for Trizol/Qiazol reagent) |
| Aplikasi | RT-PCR, Hibridisasi Utara, poly (A) enrichment, dll.. |
| Teknologi pemurnian | Acid phenol guanidine isothiocyanate |
| Metode proses | petunjuk (sentrifugasi) |
| Jenis sampel | Jaringan hewan, jaringan tanaman, adherent cells, suspended cells, bakteri, trace fungi, dll.. |
| Jumlah sampel | Flexible |
| Volume elusi | Variation with sample size |
| Waktu per lari | Variation with sample size |
Keuntungan
- Extensive – suitable for various kinds of biological samples, including animals, tanaman, sel yang dikultur, bakteri, dll..
- Hasil tinggi – efficient cleavage releases more RNA
- Kemurnian tinggi – purified RNA is suitable for various downstream applications
- Flexible – sample amount can be adjusted according to the demand
- High cost performance compared with similar products
Penyimpanan dan Stabilitas
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerjanya.
Data Eksperimen
Ulasan
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