HiPure 토탈 RNA 키트 50T- IVD4121

소개

This product offers a fast and reliable method to isolate RNA, miRNA 포함, from a wide range of samples:

• 조직
• 세포
• 피
• Other clinical samples

The extracted RNA is suitable for various downstream applications, 포함:

• RT-PCR (Reverse Transcription PCR)
• Quantitative RT-PCR (qRT-PCR)
• Viral RNA detection
• Other RNA-based analyses

This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.

명세서

원칙

• 정제방법: Silica column purification is employed in this product for effective nucleic acid extraction.
• Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
• 샘플 용해 및 소화: The sample lysis process, coupled with proteinase K digestion, takes only 15 분, ensuring rapid processing.
• Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
• Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
• Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
• 용출: 마지막으로, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.

장점

• 효율적인 DNA 제거 – 원스텝 RNA 추출 genomic DNA를 효과적으로 제거할 수 있습니다.
• 고품질 – 원스텝 RNA 추출 시약과 실리카겔 컬럼을 결합하면 최고 농도를 얻을 수 있습니다.
• 빠른 – the whole extraction only takes 15-25 분
• Nontoxic – no toxic phenol-chloroform extraction is required in the extraction

키트 내용물

보관 및 안정성

• 단백질 제 K 저장: Store Proteinase K at 2–8°C upon arrival. 단기 보관 (까지 8 주) 실온에서 (15-25°C) is acceptable without affecting its performance.
• DNase I Storage: Store DNase I at -20°C. 단기 보관 (까지 1 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다.
• Remaining Kit Components Storage: 나머지 키트 구성품은 실온에서 보관 가능 (15-25°C) 그리고 적어도 안정적으로 유지 18 이런 조건에서 몇 달 동안.