MagPure Blood DNA プレキャスト キット (96 チャンネルマシン)

導入

This product offers a convenient and rapid method to purify total DNA from various samples, making it ideal for PCR and Southern blotting applications.

• 高速で簡単な浄化: The protocol is streamlined for efficient DNA extraction with minimal hands-on time.
• High-Quality DNA: The system isolates total DNA (ゲノム, バイラル, ミトコンドリア) with high purity, suitable for reliable PCR and Southern blotting.
• Broad Sample Compatibility: The method effectively purifies DNA from a wide range of sources, 含む:
  • 全血
  • プラズマ
  • 血清
  • Buffy coat
  • Bone marrow
  • Other body fluids
  • Lymphocytes
  • Cultured cells
• Pre-packed Reagents: For user convenience, all necessary reagents are pre-plated in a 96-well plate, minimizing preparation time and ensuring consistency across samples.

仕様

原理

• Sample Lysis and Digestion: サンプルは、溶解物とプロテアーゼによって促進される溶解と消化を受けます, DNAを溶解物に放出します.
• DNA Adsorption: Magnetic particles and binding solution are added, facilitating the adsorption of DNA onto the surface of magnetic particles. Impurities such as proteins are not adsorbed.
• Washing to Remove Impurities: Adsorbed particles are washed with a washing solution to remove proteins and impurities, ensuring purity.
• Ethanol Wash: Further washing with ethanol removes salts from the particles.
• DNA Elution: DNA is eluted from the particles using an Elution Buffer, providing purified DNA for downstream applications.

利点

• 高い結合力 – DNAが豊富なサンプルの処理に適しています, 全血など, バフィーコート, 濃縮された血液, 等.
• 速い – 多分散磁気ビーズ, 高速磁気応答, そして抽出時間も短い
• 高純度 – 得られたDNAは第2世代シーケンスに直接使用できます。, PCR ベースの検出, 遺伝子バンク, 等.
• 自動 – 時間と労力を節約し、より安全に

キット内容

保管と安定性

最適なパフォーマンスのために:

• Store Proteinase K and MagPure Particles at 2-8°C (冷蔵庫) upon arrival.

Acceptable short-term storage:

• Proteinase K and MagPure Particles can be stored for up to 24 weeks at room temperature (15-25℃) without affecting their performance.

Remaining kit components:

• Store all other components dry at room temperature (15-25℃).
• They are stable for at least 18 この状況下で数か月.

Storing the entire kit:

• The entire kit can be stored at 2-8°C for long-term storage.
• If storing the entire kit cold, ensure buffers are redissolved before use.
• Always warm all buffers to room temperature before using them.