HIPURE循環DNAキット

導入

• ターゲット: Short DNA fragments (<1000血圧) in the blood (tumor markers, fetal DNA) or urine.
• 効率: Purifies circulating nucleic acids from human biofluids (プラズマ, 血清, 尿).
• アプリケーション: Extracted DNA is suitable for approved clinical diagnostic tests.

利点:

• Optimized for short DNA analysis.
• Works with various human fluids.
• A larger format processes more samples (potentially higher DNA yield).
• Directly usable in clinical tests.

This Midi Kit empowers researchers and clinical labs for applications in oncology, スキュー, and liquid biopsies.

仕様

原理

This product offers a streamlined method for isolating DNA using silica column technology:

1. 溶解と消化: サンプルは特別な解決策で治療を受けます (溶解物) と酵素 (プロテアーゼ) 開いた細胞を壊し、タンパク質を分解します, DNAを溶解物に放出します.
2. Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
3. 洗浄: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
4. 溶出: A low-salt buffer is used to elute (リリース) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

Key Advantages:

• Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
• High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (PCR, 等).
• 簡素化されたワークフロー: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

利点

• 高収量 – most optimal process, free DNA (>50血圧) can be obtained to the maximum extent
• 高濃度 – low elution volume, ensuring high nucleic acid concentration
• 高純度 – low alcohol binding method, completely removing inhibitor and protein pollution
• 高い回復力 – DNA can be recovered at the level of PG by silica gel column purification

キット内容

保管と安定性

• プロテイナーゼK & RNA Carrier: Fridge (2-8℃) for best results.
• Other Kit Components: 室温 (15-25℃) is good.
• Storing Cold? Warm buffers up before use.

実験データ