introduzione
- Target: Short DNA fragments (<1000p.b) in the blood (tumor markers, fetal DNA) or urine.
- Efficienza: Purifies circulating nucleic acids from human biofluids (plasma, siero, urine).
- Applicazioni: Extracted DNA is suitable for approved clinical diagnostic tests.
Benefici:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolamento del DNA circolante da 1-5 ml di plasma, siero, and body fluids using vacuum protocol |
| Applicazioni | qPCR, liquid or solid chip analysis, hybridization SNP detection, eccetera. |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (vacuum) |
| Tipo di campione | Siero, plasma, and other cell-free fluid samples |
| Importo del campione | 1-5ml |
| Volume di eluizione | ≥50 μl |
| Tempo per corsa | ≤60 minuti |
| Volume di trasporto del liquido per colonna | 4ml |
| Resa legante della colonna | 1mg |
Principio
This product offers a streamlined method for isolating DNA using silica column technology:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, Rilasciare il DNA nel lisato.
- Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
- Washing: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer is used to elute (release) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Key Advantages:
- Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (PCR, eccetera.).
- Flusso di lavoro semplificato: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Vantaggi
- Alta resa – processo più ottimale, free DNA (>50p.b) can be obtained to the maximum extent
- High concentration – low elution volume, ensuring high nucleic acid concentration
- Purezza elevata – low alcohol binding method, completely removing inhibitor and protein pollution
- Recupero elevato – DNA can be recovered at the level of PG by silica gel column purification
Contenuto del kit
| Contenuti | IVD3182 |
| Tempi di purificazione | 50 |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinasi K | 540 mg |
| Tampone di dissoluzione della proteasi | 30 ml |
| RNA portante | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml Provette di raccolta | 100 |
| Extender Tube | 50 |
| Vac-Connector | 50 |
Conservazione e stabilità
- Proteinasi K & RNA Carrier: Fridge (2-8°C) for best results.
- Other Kit Components: Temperatura ambiente (15-25°C) is good.
- Storing Cold? Warm buffers up before use.
Dati dell'esperimento
Recensioni
Non ci sono ancora recensioni.