- Descrizione del prodotto:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Sample Types:
- Sangue intero
- Cappotto Buffy
- Midollo osseo
- Body fluids
- Purification Time: As little as 25 minuti.
- Caratteristiche:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from whole blood using the economic salt out method |
| Applicazioni | PCR, enzyme digestion, Southern hybridization, eccetera. |
| Tecnologia di purificazione | Salting out method |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto animale |
| Importo del campione | Unlimited |
| Volume di eluizione | ≥100μl |
| Tempo per corsa | Variation with sample amount |
Principles
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- Rimozione dei contaminanti:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 E 1.9.
- DNA size can reach up to 200 kb.
Vantaggi
- Sicuro – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Purezza elevata – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Contenuto del kit
| Contenuti | D331101 | D331102 | D331103 |
| Purification Volumes | 50 ml | 300 ml | 1000 ml |
| 10 x Buffer RBC | 20 ml | 100 ml | 3 X 100 ml |
| Buffer WTL | 60 ml | 350 ml | 1000 ml |
| Buffer PPS | 20 ml | 120 ml | 350 ml |
| Soluzione di RNasi | 300 ml | 1.8 ml | 6 ml |
| Buffer TE | 10 ml | 30 ml | 120 ml |
Conservazione e stabilità
- RNase Solution Storage:
- Upon arrival, store at 2-8°C.
- Short-term storage (fino a 24 settimane) a temperatura ambiente (15-25°C) does not affect performance.
- Remaining Kit Components:
- Dry components can be stored at room temperature (15-25°C).
- Stable for at least 18 mesi in queste condizioni.
- Entire Kit Storage:
- The entire kit can be stored at 2-8°C.
- Buffers should be redissolved before use if stored at this temperature.
- Ensure all buffers are at room temperature when used.
