- Descripción del Producto:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Sample Types:
- Sangre pura
- Buffy coat
- Bone marrow
- Body fluids
- Purification Time: As little as 25 minutos.
- Características:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of total DNA from whole blood using the economic salt out method |
| Aplicaciones | PCR, digestión enzimática, Southern hybridization, etc.. |
| Tecnología de purificación | Salting out method |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Tejido animal |
| Cantidad de muestra | Unlimited |
| Volumen de elución | ≥100μl |
| Tiempo por carrera | Variation with sample amount |
Principios
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- Eliminación de contaminantes:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 y 1.9.
- DNA size can reach up to 200 kb.
Ventajas
- Seguro – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, no tóxico, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Alta pureza – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Contenido del kit
| Contenido | D331101 | D331102 | D331103 |
| Purification Volumes | 50 ml | 300 ml | 1000 ml |
| 10 x Buffer RBC | 20 ml | 100 ml | 3 X 100 ml |
| Buffer WTL | 60 ml | 350 ml | 1000 ml |
| Buffer PPS | 20 ml | 120 ml | 350 ml |
| Solución de ARNasa | 300 l | 1.8 ml | 6 ml |
| Buffer TE | 10 ml | 30 ml | 120 ml |
Almacenamiento y estabilidad
- RNase Solution Storage:
- A llegado, almacenar a 2-8 ° C.
- Almacenamiento a corto plazo (hasta 24 semanas) a temperatura ambiente (15-25°C) no afecta el rendimiento.
- Componentes del kit restantes:
- Dry components can be stored at room temperature (15-25°C).
- Estable por al menos 18 meses en estas condiciones.
- Entire Kit Storage:
- Todo el kit se puede almacenar a 2-8°C..
- Buffers should be redissolved before use if stored at this temperature.
- Asegúrese de que todos los buffers estén a temperatura ambiente cuando se usan.
