Bilirubina diretta Solarbio (DBIL) Kit di analisi 50T | 48S

Nota: Prendi due o tre campioni diversi per la previsione prima del test.

Attrezzatura operativa: Spettrofotometro/lettore di micropiastre

Numero di catalogo: BC5175

Misurare: 50T | 48S

Componenti:

Reagente I: 25 ml×1. Store at 2-8℃.

Reagente II: 6 ml×1. Store at 2-8℃.

Descrizione del prodotto：

Bilirubina diretta (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 nm. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 nm.

Reagenti e attrezzature necessari ma non forniti：

Spettrofotometro/lettore di micropiastre, centrifuga da tavolo, constant temperature foster box/water bath, pipette, microcuvetta in vetro/piastra a fondo piatto da 96 pozzetti, ghiaccio, e acqua distillata.

Procedura

UN. preparazione del campione:

Siero, plasma, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

B.Determinazione procedura：

1. Preheat the spectrophotometer/microplate reader for 30min, regolare la lunghezza d'onda su 450 nm, and set the spectrophotometer counter to zero with distilled
2. Determinazione:

Reagente (μL)	Provetta	Tubo vuoto
Campione	8	–
Acqua distillata	–	8
Reagente I	192	192
Mescolare bene. React at 37℃ for 5 minuti. Misurare l'assorbanza a 450 nm, and record it as A1T, A1b;
Reagente II	48	48
Mescolare bene. React at 37℃ for 5 minuti. Misurare l'assorbanza a 450 nm, and record it as A2T, A2B. Calculate the ΔAT =A1T-A2T and the ΔAB =A1B-A2B. Blank tubes only need to be measured once or twice.

C.Calcoli：

96 piastra a fondo ben piatto

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343

Micro glass cuvetta

DBIL content (μmol/L) =1099.7×(ΔAT-ΔAB)-10.73

Nota:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, si consiglia di diluire il campione prima della determinazione.

Esempi:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 piastra a fondo ben piatto, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343=54.95 μmol/L.

Prodotti correlati

BC5180/BC5185 Total bilirubin (TBIL) Kit di analisi del contenuto