introduzione
The Kit integrates phenol/guanidina-based lysis and silica-membrane purification of total RNA. Reagente MagZol, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, pianta, and fungal samples, batteri, cellule, e altri campioni.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total RNA (including miRNA) from 100mg lipid tissue, tessuto, cella, pianta, and body fluids using columns and MagZol reagent |
| Applicazioni | RT-PCR, qRT-PCR, Northern hybridization, second-generation sequencing |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto animale, muscle fiber-containing tissue, adipose tissue, cellule coltivate, linfociti, simple plants, and other biological samples |
| Importo del campione | Animal tissue sample: 1-60mg (spleen ≤ 10mg)
Cellule coltivate: 5 X 106 Plant leaves: 50-100mg |
| Prodotto | 2-200μg |
| Volume di eluizione | ≥50 μl |
| Tempo per corsa | ≤30 minutes（1-24 samples） |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100mg |
Principio
Campioni di tessuto (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Vantaggi
- Efficient DNA removal – One-step Estrazione dell'RNA can effectively remove genomic DNA
- Alta qualità – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Veloce – possono essere estratti diversi campioni 30 minuti
- High applicability – samples including animals, impianti, batteri, cellule, eccetera.
- High output – enables to processing of large samples and the yield can be up to 200μg
Contenuto del kit
| Contenuti | R413002 | D413003 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Provette di raccolta | 50 | 250 |
| Reagente MagZol | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 50 ml |
| Acqua libera da RNasi | 10 ml | 30 ml |
Conservazione e stabilità
MagZol Reagent should be stored at 2-8°C upon arrival. Tuttavia, conservazione a breve termine (fino a 12 settimane) a temperatura ambiente (15-25°C) does not affect its performance. I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni.
Dati dell'esperimento
Recensioni
Non ci sono ancora recensioni.