Fast and Easy Total Pemurnian DNA
- Objective: Reliable PCR and Southern Blotting
- Fitur:
- Swift purification methods
- Easy-to-follow protocols
- Manfaat:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Seluruh darah
- Plasma
- Serum
- Buffy Coat
- Sumsum tulang
- Cairan tubuh lainnya
- Limfosit
- Sel yang dikultur
- Purifies various types of total DNA:
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from 200μl whole blood |
| Aplikasi | PCR, deteksi baut selatan dan virus, dll. |
| Teknologi pemurnian | Teknologi manik-manik magnetik |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Seluruh darah, darah terkonsentrasi, mantel buffy, dan sampel lainnya |
| Jumlah sampel | 200μl |
| Volume elusi | ≥70μl |
| Waktu per lari | ≤40 minutes |
Prinsip
- Sample lysis and digestion facilitated by lysate and Protease.
- DNA dilepaskan ke dalam lisat.
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
Prosedur:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: DNA dilepaskan ke dalam lisat.
- DNA adsorption: Partikel magnetik dan larutan pengikat ditambahkan, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Pencucian:
- Pertama, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Elusi: DNA is eluted using an Elution Buffer.
Keuntungan:
-
- High specificity: Selective DNA binding, excluding impurities.
- Efisiensi: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Aplikasi:
-
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Keuntungan
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, seperti darah utuh, mantel buffy, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- DNA dengan Kemurnian Tinggi: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Urutan generasi kedua (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Isi Paket
| Isi | D631001 | D631002 | D631003 |
| Waktu Pemurnian | 48 | 96 | 480 |
| Partikel MagPure | 1.7 ml | 3.5 ml | 17 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Buffer Larut Protease | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Buffer DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Penyangga Elusi | 15 ml | 30 ml | 100 ml |
Penyimpanan dan Stabilitas
Proteinase K, MagPureParticles should be stored at 2-8°C upon arrival. Namun, penyimpanan jangka pendek(hingga 24 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15-25°C)dan setidaknya stabil 18 bulan dalam kondisi ini.
