Fast and Easy Total DNA-Reinigung
- Objektiv: Reliable PCR and Southern Blotting
- Merkmale:
- Swift purification methods
- Easy-to-follow protocols
- Vorteile:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Vollblut
- Plasma
- Serum
- Buffy-Mantel
- Knochenmark
- Andere Körperflüssigkeiten
- Lymphozyten
- Kultivierte Zellen
- Purifies various types of total DNA:
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from 200μl whole blood |
| Anwendungen | PCR, Südlicher Bolzen und Virenerkennung, usw |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | Vollblut, konzentriertes Blut, Buffy-Mantel, und andere Proben |
| Probenmenge | 200μl |
| Elutionsvolumen | ≥70μl |
| Zeit pro Lauf | ≤ 40 Minuten |
Prinzip
- Sample lysis and digestion facilitated by lysate and Protease.
- DNA wird in das Lysat freigesetzt.
- DNA adsorption onto magnetic particles‘ surface, excluding impurities like proteins.
Verfahren:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: DNA wird in das Lysat freigesetzt.
- DNA adsorption: Magnetpartikel und Bindungslösung werden zugesetzt, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Waschen:
- Erste, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Elution: DNA is eluted using an Elution Buffer.
Vorteile:
-
- High specificity: Selective DNA binding, excluding impurities.
- Effizienz: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Anwendungen:
-
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Vorteile
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, wie Vollblut, Buffy-Mantel, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Sequenzierung der zweiten Generation (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Inhalt des Kits
| Inhalt | D631001 | D631002 | D631003 |
| Reinigungszeiten | 48 | 96 | 480 |
| Elsträgerpartikel | 1.7 ml | 3.5 ml | 17 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease-Auflösungspuffer | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Puffer DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Elutionspuffer | 15 ml | 30 ml | 100 ml |
Lagerung und Stabilität
Proteinase K, MagPureParticles should be stored at 2-8°C upon arrival. Jedoch, kurzfristige Lagerung(bis zu 24 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15-25°C)und sind mindestens stabil 18 Monate unter diesen Bedingungen.
