The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tisu, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, Poly A+ RNA (mRNA) pemurnian, perlindungan nuclease, dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| Aplikasi | RT-PCR, qRT-PCR, Hibridisasi Utara, urutan generasi kedua |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Animal soft tissue, sel yang dikultur, limfosit, simple plant tissue |
| Jumlah sampel | Sel: ≤1x 107
Jaringan hewan: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 X 106 |
| Menghasilkan | 2-100μg |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤15 minutes（1-24 samples） |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
Teknologi Hipure RNA menyederhanakan isolasi RNA total. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Keuntungan
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – beberapa sampel dapat diekstraksi 20 Metode menit demi kolom
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Peka – RNA dapat dipulihkan pada tingkat PG
Isi Paket
| Isi | R401102 | R401103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Kolom Mini RNA HiPure | 50 | 250 |
| 2ml Tabung Koleksi | 50 | 250 |
| Penyangga Lisis RTL | 50 ml | 200 ml |
| RNA Binding Buffer | 15 ml | 75 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Penyangga RW2* | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
The Kit can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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