The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA (mRNA) pročišćavanje, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija Ukupna RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| Prijave | RT-PCR, qRT-PCR, Sjeverna hibridizacija, second generation sequencing |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Količina uzorka | Cells: ≤1 x 107
Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 x 106 |
| Prinos | 2-100μg |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤15 minutes（1-24 samples） |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Prednosti
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Brzo – može se izdvojiti nekoliko uzoraka 20 minutes by column method
- Sef – no phenol chloroform extraction required
- Osjetljiv – RNA can be recovered at the level of PG
Sadržaj kompleta
| Sadržaj | R401102 | R401103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 250 |
| RTL Lysis Buffer | 50 ml | 200 ml |
| RNA Binding Buffer | 15 ml | 75 ml |
| Međuspremnik RW1 | 50 ml | 200 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
Još nema recenzija.