Perkenalan
Produk ini merupakan bahan baku inti untuk ekstraksi asam nukleat setelah penyaringan dan kontrol kualitas yang ketat. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.
Detail
Spesifikasi
| Fitur | Spesifikasi | |
| Konten dasar | Aplikasi yang direkomendasikan | Agarose Gel Pemurnian DNA, DNA/RNA Isolation (Lysis Buffer) |
| Nomor CAS | 593-84-0 | |
| Rumus | CH5N3•HSCN | |
| Berat molekul | 118.6 | |
| Isi | 99.5% | |
| Tingkat | Biologi Molekuler | |
| Penampilan | White powder to crystal | |
| Kondisi transportasi | Suhu kamar, kering | |
| Kondisi pelestarian | Avoid light | |
| Parameter pengotor | kelembaban | ≤0,3% |
| Abu | ≤0,02% | |
| Materi yang tidak larut dalam air | ≤0,02% | |
| Amonium | ≤0,05% | |
| Amonium disianida | ≤0,1% | |
| Amonium sianurat klorida | ≤0,01% | |
| Besi | ≤0.1PPM | |
| Nilai serapan UV | Nilai serapan @ 230 (6M) | ≤20 |
| Nilai serapan @ 260 (6M) | ≤25 | |
| Nilai serapan @ 280 (6M) | ≤0.3 | |
| Nilai serapan @ 320 (6M) | ≤0,01 | |
| Terkait ekstraksi asam nukleat | Gel DNA Purification | Mengambil |
| RNA Isolation | Mengambil | |
| tes DNAse (1M) | Tidak terdeteksi | |
| tes RNase (1M) | Tidak terdeteksi | |
| Kemurnian larutan jenuh (6M) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| nilai PH (1M) | 5.0-7.0 |
Meminta informasi
| KUCING.Tidak. | Nama Produk | Kemasan |
| C11103 | Guanidine Thiocyanate
(Biologi Molekuler) |
5kg |
| C11104 | 25kg (5KG/Tas, 5Tas/Ember) |
Ulasan
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