Uvod
Proizvod je jezgrena sirovina za ekstrakciju nukleinskih kiselina nakon strogog probira i kontrole kvalitete. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.
pojedinosti
Tehnički podaci
| Značajke | Specifikacija | |
| Basic content | Preporučena prijava | Agarose Gel Pročišćavanje DNK, DNA/RNA Isolation (Lysis Buffer) |
| CAS brojevi | 593-84-0 | |
| Formula | CH5N3•HSCN | |
| Molekularna masa | 118.6 | |
| Content | 99.5% | |
| Nivo | Molekularna biologija | |
| Izgled | White powder to crystal | |
| Transportation conditions | Room temperature, dry | |
| Uvjeti očuvanja | Avoid light | |
| Impurity parameters | Moisture | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤20 |
| Absorbance value @ 260 (6M) | ≤25 | |
| Absorbance value @ 280 (6M) | ≤0.3 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Gel DNA Purification | Adopt |
| RNA izolacija | Adopt | |
| DNase test (1M) | Nije otkriveno | |
| RNase test (1M) | Nije otkriveno | |
| Purity of saturated solution (6M) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| PH value (1M) | 5.0-7.0 |
Informacije o naručivanju
| Mačka.no. | Naziv proizvoda | Paket |
| C11103 | Guanidine Thiocyanate
(Molekularna biologija) |
5Kg |
| C11104 | 25Kg (5KG/Bag, 5Bags/Bucket) |
Recenzije
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