HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sekvenciranje, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
| Prijave | PCR, sekvenciranje, labeling reactions, ligations and restriction digestion, itd. |
| Metoda pročišćavanja | 96 well Bind Plate |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | PCR product, enzymatic reaction |
| Količina uzorka | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>Prikladno |
| Oporavak | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥80% |
| Volumen eluacije | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥75μl |
| Vrijeme po vožnji | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤60 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 20µg |
Načelo
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Prednosti
- High recovery efficiency – ≥80% DNA recovery
- Brzo – isolation can be completed in 60 minuta
Sadržaj kompleta
| Sadržaj | D212201 | D212202 | D212203 |
| Vremena pročišćavanja | 1 x 96 Pripreme | 4 x 96 Pripreme | 20 x 96 Pripreme |
| Buffer GDP | 120 ml | 400 ml | 4 x 400 ml |
| Buffer DW2 | 20 ml | 100 ml | 3 x 100 ml |
| Elucijski pufer | 20 ml | 20 ml | 30 ml |
| HiPure DNA Plate | 1 | 4 | 20 |
| 2.2ml Collection Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.8 ml Collection Plate | 1 | 4 | 20 |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Ako se u puferima stvori bilo kakav talog, zagrijte na 37 ℃ da se otopi.
Recenzije
Još nema recenzija.