Uvod
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Sjeverna hibridizacija, itd. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, itd.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from bacteria culture |
| Prijave | RT-PCR, Northern blot, poly-A + pročišćavanje, nucleic acid protection and in vitro translation |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Bacteria culture |
| Količina uzorka | Bacteria: <10^9 |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤40 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as gvanidin hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 30 minuta
- Visoka čistoća – the purified RNA can be directly used in various downstream applications
- Visoki oporavak – RNA can be recovered at the level of PG
- Dobra ponovljivost – Tehnologija pročišćavanja stupca silikagela može svaki put dobiti idealne rezultate
- Široki spektar – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried lysozyme and glass beads
Sadržaj kompleta
| Sadržaj | R418102 | R418103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 250 |
| Glass Beads (0.1-0.2mm) | 20 g | 90 g |
| Lizocim | 90 mg | 400 mg |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Buffer STE | 10 ml | 30 ml |
| MagZol Reagent | 60 ml | 270 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2-8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
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