spécification
| Attribut | Détails |
|---|---|
| Stockage | 2-8℃ |
| Unité | Box |
| Validity Period | 6 mois |
| Nom anglais | Pepsase Activity Assay Kit |
| Méthode de détection | Spectrophotomètre/lecteur de microplaques |
| spécification | 100T/48S |
Product Composition
| Nom du réactif | spécification | Storage Condition |
|---|---|---|
| Solution d'extraction | Liquide 50 mL×1 bouteille | Conserver à 4℃ |
| Réactif | Powder×1 bottle | Conserver à 4℃ |
| Réactif deux | Liquide 15 mL×1 bouteille | Conserver à 4℃ |
| Réactif trois | Powder×1 bottle | Conserver à 4℃ |
Instructions for preparing the solutions:
- Solution One: Ajouter 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: Ajouter 10 mL of distilled water before use and dissolve completely.
Description du produit:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- Eau distillée
Note: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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