MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) et les cellules (<5 X106), and large quantities of tissue (up to 1g) et les cellules (<108), of human, animal, usine, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(UN) + sélection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | RNA isolation solvent (substitution for Trizol/Qiazol reagent) |
| Applications | RT-RAP, Northern hybridization, poly (un) enrichment, etc.. |
| Technologie de purification | Acid phenol guanidine isothiocyanate |
| Méthode de processus | Manuel (centrifugation) |
| Échantillon type | Tissu animal, tissu végétal, adherent cells, suspended cells, bactéries, trace fungi, etc.. |
| Montant de l'échantillon | Flexible |
| Volume d'élution | Variation with sample size |
| Temps par course | Variation with sample size |
Avantages
- Extensive – suitable for various kinds of biological samples, including animals, plantes, cultured cells, bactéries, etc..
- Haut rendement – efficient cleavage releases more RNA
- Haute pureté – purified RNA is suitable for various downstream applications
- Flexible – sample amount can be adjusted according to the demand
- High cost performance compared with similar products
Stockage et stabilité
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. Cependant, stockage à court terme (jusqu'à 12 semaines) à température ambiante (15-25°C) does not affect its performance.
Données d'expérimentation
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