This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-RAP, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from tissue and cells and plant |
| Applications | RT-PCR, synthèse d'ADNc, second generation sequencing |
| Méthode de purification | Billes magnétiques polydispersées |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel ou automatique |
| Instrument adaptatif | Extracteur d'acide nucléique, poste de travail de pipetage |
| Échantillon type | Tissu animal, human tissue, cultured cells, lymphocytes, ordinary plant tissue |
| Montant de l'échantillon | Cellules: 1 X 107
Tissu animal: ≤20mg Plant tissue: ≤100mg Yeast cell: ≤5 x 106 |
| Rendement | 5-100µg |
Principe
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Avantages
- Haute pureté – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
- Économie – moins que 50% du prix de Qiagen et d'autres produits importés
- Automatique – extraction without manual participation, saving time and effort
- Universal – suitable for various animal tissues, cultured cells and conventional plant fungal tissues
Contenu du kit
| Contenu | R662201 | R662202 | R662203 |
| Temps de purification | 48 Préparations | 96 Préparations | 5 X 96 Préparations |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| Protéinase K | 24 mg | 50 mg | 240 mg |
| Tampon de dissolution de protéase | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 µl | 2 X 600 µl | 10 X 600 µl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Tampon de lyse RTL | 40 ml | 80 ml | 400 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Tampon RW2* | 20 ml | 50 ml | 2 X 100 ml |
| Eau sans RNase | 10 ml | 30 ml | 120 ml |
Stockage et stabilité
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. Cependant, stockage à court terme (DNase I up to 1 semaines, MagPure RNA Particles and Proteinase K up to 8 semaines) à température ambiante (15–25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être conservés à température ambiante (15–25°C) and are stable for atleast 18 mois dans ces conditions.
Commentaires
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