MagPure A4 XP Features:
- Technology Base:
- Employs Magen’s solid-phase paramagnetic bead technology designed for the purification of RAP amplicons.
- High-Throughput Capability:
- Suitable for high-throughput settings, allowing for efficient simultaneous processing of multiple samples.
AmPure Technology:
- Liaison sélective:
- Uses an optimized buffer that selectively binds PCR amplicons 100bp and larger to paramagnetic beads.
- Élimination des contaminants:
- Simple washing procedure efficiently removes excess primers, nucléotides, sels, and enzymes.
- Purity of PCR Product:
- Results in PCR products that are virtually free from contaminants, suitable for precise downstream applications.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
| Applications | NGS, DNA library |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | DNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Montant de l'échantillon | Appropriate |
| Récupération | 80% |
| Temps de fonctionnement | ≤50 minutes |
Principe
Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Élimination des contaminants:
- Excess primers, nucléotides, sels, and enzymes are removed through a simple washing procedure.
- Élution d'ADN:
- Purified DNA is eluted using Elution Buffer or Water.
Avantages
- High recovery — up to 80%
- High throughput — using magnetic beads purification technology
Contenu du kit
| Contenu | BP-5 | BP-50 | BP-500 |
| MagPure A4 XP | 5 ml | 50 ml | 500 ml |
Stockage et stabilité
Storage and Handling Instructions:
- Conditions de stockage:
- Store MagPure A4 XP at 2-8°C upon arrival.
- Stable for up to 18 mois dans ces conditions.
- Short-term Storage:
- Stockage à court terme (jusqu'à 4 semaines) à température ambiante (15-25°C) n'affecte pas les performances.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogeneous and consistent in color.
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