Introduction
- But: Extraction of stool RNA.
- Compatibilité des échantillons: Suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples.
- Technologie: Utilizes silica gel column purification technology and an original solution system.
- Key Feature: Effectively removes humic acid and other inhibitory factors present in stool samples.
- Applications: The purified RNA can be directly used in RT-RAP, Northern hybridization, and other experiments.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from 100-150mg stool sample |
| Applications | RT-PCR, Northern hybridization, and other experiments |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Stool |
| Montant de l'échantillon | 100-150 mg |
| Volume d'élution | ≥30 μl |
| Temps par course | ≤50 minutes |
| Volume de liquide transporté par colonne | 100µg |
| Rendement de liaison de la colonne | 800µl |
Principe
- Principle of HiPure Silica Gel Column: Utilizes a high binding ability glass fiber filter membrane as the substrate. Under high concentrations of ionizing agents (par exemple., Guanidinium chloride or guanidine isothiocyanate), nucleic acids are adsorbed through hydrogen bonding and electrostatic interactions, while proteins and other impurities are not adsorbed and removed. Ensuite, the filter membrane with adsorbed nucleic acids is washed to remove proteins and salts. Enfin, low salt buffer solution (par exemple., Tampon) or water is used to elute the purified nucleic acids, resulting in high purity suitable for downstream experiments.
- Traitement des échantillons: Stool samples are homogenized in the lysis solution, then further lysed in a high-temperature water bath to release RNA into the solution. Chloroform extraction is employed to remove genomic DNA and impurities. The supernatant is transferred to an alcohol-free binding solution, followed by RNA purification through a column. Enfin, RNA is eluted using RNase Free Water.
- Applications: The purified RNA is suitable for various downstream experiments such as PCR, Hybridation du Sud, and enzyme digestion.
Avantages
- Haute pureté: Utilizes a unique adsorbent for more efficient removal of inhibitors, ensuring high purity of extracted RNA.
- High Concentration: Achieves maximum extraction of total RNA from stool samples due to its high concentration, maximizing RNA yield.
- Sensible: Capable of purifying RNA at the level of picograms (PG), ensuring sensitivity in downstream applications.
Contenu du kit
| Contenu | R418502 | R418503 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure ARN | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| Glass Beads (0.1~0.6mm) | 30 g | 150 g |
| Buffer SPL | 30 ml | 140 ml |
| Buffer PHC | 30 ml | 140 ml |
| Buffer GRP | 60 ml | 250 ml |
| Tampon RW1 | 50 ml | 250 ml |
| Buffer RW2 * | 20 ml | 2 X 50 ml |
| Eau sans RNase | 15 ml | 30 ml |
Stockage et stabilité
- Conditions de stockage: Kit components can be stored at room temperature (15–25°C) and remain stable for 18 mois dans ces conditions.
- Buffer SPL: May form precipitates at low temperatures. Dissolve precipitates by placing the Buffer SPL in a 55°C water bath.
- Buffer PHC: After receiving the product, store Buffer PHC at 2-8°C.
Commentaires
Il n'y a pas encore de critiques.