This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. L'ADN peut être utilisé directement pour RAP, PCR quantitative, southern Blot, Test de l'ADN virus et ainsi de suite.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| Applications | RAP, SSR, AFLP, RAPD and southern blot, etc.. |
| Méthode de purification | 96 bien plaque |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Economic plant samples |
| Montant de l'échantillon | Fresh / frozen plant samples: ≤50mg
Dried plant / seed samples: ≤15mg |
| Volume d'élution | ≥75μl |
| Temps par course | 30-50 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Avantages
- High quality DNA – meet a variety of downstream applications, y compris PCR, qPCR, digestion enzymatique, blot hybridization, etc..
- Débit élevé – 96 samples can be processed simultaneously by 96 bien plaque
- High permeability – using composite membrane technology, no hole plugging
Contenu du kit
| Contenu | D316702 | D316703 |
| Temps de purification | 4 X 96 Préparations | 20 X 96 Préparations |
| Solution de RNase | 5 ml | 22 ml |
| Tampon d'élution | 120 ml | 500 ml |
| Buffer SPL | 200 ml | 2 X 500 ml |
| Buffer PS | 100 ml | 400 ml |
| Tampon GW1 | 220 ml | 5 X 220 ml |
| Tampon GW2 | 100 ml | 3 X 100 ml |
| 1.6ml Collection Plate | 4 | 20 |
| HiPure gDNA Plate | 4 | 20 |
| 2.2ml Collection Plate(DW Plate) | 8 | 40 |
| 0.8ml Collection Plate(DW Plate) | 4 | 20 |
| Sealing Film | 20 | 100 |
Stockage et stabilité
RNase solution should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 24 semaines) à température ambiante (15-25°C) does not affect its performance. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Guide d'achat
| Nom | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Volume d'élution | Rendement (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100µl | 3-70µg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250µg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100µl | 3-50µg |
| HiPure SF Plant DNA 96 Trousse | D3167 | 50mg | 15mg | 96 bien plaque | 75 – 150µl | 3-30µg |
Commentaires
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