HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total Extraction d'ARN. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (y compris les miARN) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-RAP, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc..
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ARN total (include miRNA) depuis <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes) |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea |
| Montant de l'échantillon | ≤200 mg |
| Volume d'élution | ≥30 μl |
| Temps par course | 1-24 samples within 30 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
Avantages
- Completely remove DNA by using of DNase
- Haute qualité – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Rapide – Plusieurs échantillons peuvent être extraits dans 40 minutes by column method
- Sensible – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Contenu du kit
| Contenu | R416502 | D416503 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure ARN | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 250 |
| DNase I | 600 µl | 5 X 600 µl |
| DNase Buffer | 6 ml | 30 ml |
| Buffer PAL | 60 ml | 270 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer BDP | 60 ml | 270 ml |
| Tampon RW1 | 50 ml | 250 ml |
| Tampon RW2* | 20 ml | 2 X 50 ml |
| Eau sans RNase | 10 ml | 30 ml |
Stockage et stabilité
DNase I should be stored at -20-8°C upon arrival. Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. L'ensemble du kit peut être conservé entre 2 et 8°C, mais dans ce cas, les tampons doivent être redissous avant utilisation. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés.
Commentaires
Il n'y a pas encore de critiques.