HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, RAP, séquençage, digestion des restrictions, or various labeling reactions. En outre, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | DNA Clean Up from agarose Gel and Reactions |
| Applications | RAP, NGS, labeling, ligation and enzyme digestion, etc.. |
| Méthode de purification | Micro colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Gel d'agarose, PCR products, enzyme products |
| Montant de l'échantillon | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>Gel d'agarose: ≤300mg
DNA products: <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≤300µl (<portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≤5µg) |
| Récupération | 100-5000pb：85%, 5-10ko：60-70% |
| Volume d'élution | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≥10μl |
| Temps par course | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≤30 minutes（1-24 samples） |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 20µg |
Principe
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Avantages
- High recovery efficiency – jusqu'à 80% DNA recovery
- Low elution volume – elution volume can be as low as 10μl
- Général – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
- Fast – isolation can be completed in 10-15 minutes by column gel method
Contenu du kit
| Contenu | D211002 | D211003 |
| Temps de purification | 100 Préparations | 250 Préparations |
| Buffer GDP | 60 ml | 125 ml |
| Buffer DW1 | 40 ml | 90 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Tampon d'élution | 20 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 Tubes de prélèvement ml | 100 | 250 |
Stockage et stabilité
The Kit should be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
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