Micro kit d'acide nucléique circulant HiPure

Micro kit d'acide nucléique circulant HiPure, is designed to efficiently purify short nucleic acids (<1000pb) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfDNA) and mRNA in blood
  • Fetal nucleic acids in maternal blood
  • Free-circulating cell-free RNA (cfRNA)
  • Viral nucleic acids
• Compatibilité des échantillons:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Efficient Purification: The kit isolates short nucleic acids present in biofluids.
• Haute pureté: Purified nucleic acids are free of proteins, nucleases, and other contaminants.
• Applications en aval: The eluted nucleic acids are ready for use in various downstream analyses, y compris:
  • Amplification reactions (par exemple., RAP)
  • Long-term storage (-30°C to -15°C)

Avantages:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Compatibilité des échantillons larges: Works effectively with various human biofluids.
• Résultats de haute qualité: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, y compris l'oncologie, non-invasive prenatal testing (NIPT), and viral diagnostics.

Caractéristiques

Principe

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Voici une ventilation du processus:

1. Lyse et digestion:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. Cela libère l'ADN dans la solution.

2. Liaison à la membrane de silice:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Lavage:

• Les tampons de lavage sont passés à travers la colonne, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Élution:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, comme PCR, Southern Blot, et séquençage de nouvelle génération.

Avantages

• Efficient Purification: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• ADN de haute qualité: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
• Flux de travail simplifié: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

Contenu du kit

Stockage et stabilité

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (réfrigérateur) upon arrival for best results.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be stored for up to 12 semaines à température ambiante (15-25°C) without affecting their performance.

Composants du kit restant:

• Stockez tous les autres composants du kit à sec à température ambiante (15-25°C).
• Ils sont stables pendant au moins 18 mois dans ces conditions.

Important Notes:

• If storing the entire kit at 2-8°C, ensure buffers are redissolved before use.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.