This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative RAP, liquid or solid phase chip analysis, hybridation, et détection SNP.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement des deux ADN/ARN circulants (include miRNA) depuis 1-5 ml de sérum et de plasma |
| Applications | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridation et détection de SNP |
| Méthode de purification | Midi colonne de rotation |
| Technologie de purification | Technologie de la silice, DNA filtration technology |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | sérum, plasma, and other cell-free liquid samples |
| Montant de l'échantillon | 1-5 ml |
| Volume d'élution | ≥20μl |
| Temps par course | ≤100 minutes |
| Volume de liquide transporté par colonne | 4 ml |
| Rendement de liaison de la colonne | 1 mg |
Principe
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. ADN / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Enfin, ADN / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, analyse de copeaux en phase liquide ou solide, hybridation et détection de SNP.
Avantages
- Haut rendement – most optimized process to obtain maximum free RNA and small RNA
- Haute concentration – faible volume d'élution (>20µl) to ensure nucleic acid concentration
- Haute pureté – low alcohol combination, completely remove inhibitor and protein pollution
- Récupération élevée – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
Contenu du kit
| Contenu | R431602 | D431603 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Micro Columns | 50 | 5 X 50 |
| HiPure Viral Midi Columns | 50 | 5 X 50 |
| 15 Tubes de prélèvement ml | 50 | 5 X 50 |
| 2Tubes de prélèvement ml | 50 | 5 X 50 |
| Buffer CFL | 150 ml | 2 X 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 X 250 ml |
| Tampon RW2* | 2 X 50 ml | 5 X 100 ml |
| Eau sans RNase | 10 ml | 50 ml |
Stockage et stabilité
The kit components can be stored at room temperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions.
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